There are five basic principles that affect visibility of an ad: color scheme, ad unit location, ad format, display type and style. Ultimately, it is visibility, relevance and other factors that affect the performance of your ad units. Among these factors are things like individual consumer mood, which is completely outside a publisher's control.
pubCenter allows you to control the look, feel and behavior of your ad units. You can customize your ad units and experiment with them to see which ad units perform best. Keep in mind that making changes can have a profound impact on ad unit performance. That impact can be positive, negative or neutral. Ultimately, experimenting with your ad units is up to you.
See the Optimization Tips in the Help Center for more details on how to optimize your ad units. It is always good to test the different optimization techniques to see how revenue is impacted.
Please check out our Publisher Community to see how other publishers successfully optimize their ad units.